ELISA/assay

Human GPX1 (Glutathione Peroxidase 1) ELISA Kit

MBS2516156

48-Strip-Wells

410 USD

ELISA Kit

Human GPX1 (Glutathione Peroxidase 1) ELISA Kit

GPX1

GPX1 protein; Glutathione peroxidase 1; glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase; glutathione peroxidase 1; Cellular glutathione peroxidase

GPX1

GPX1; GPX1; GPXD; GSHPX1; GPx-1; GSHPx-1

GPX1_HUMAN

Human

145

This kit recognizes natural and recombinant Human GPX1. No significant cross-reactivity or interference between Human GPX1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Human GPX1 is 18.75pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GPX1 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GPX1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GPX1. You can calculate the concentration of GPX1 in the samples by comparing the OD of the samples to the standard curve.

47124246

AAH70258.1

10,289 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Direct P53 Effectors Pathway (137939); Folate Metabolism Pathway (198833)

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family. Protein type: Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.11.1.9. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol. Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding. Biological Process: fat cell differentiation; response to nicotine; blood vessel endothelial cell migration; response to toxin; myoblast proliferation; skeletal muscle fiber development; negative regulation of caspase activity; thermoregulation; regulation of gene expression, epigenetic; vasodilation; response to estradiol stimulus; endothelial cell development; regulation of mammary gland epithelial cell proliferation; response to selenium ion; glutathione metabolic process; sensory perception of sound; lipoxygenase pathway; response to symbiotic bacterium; UV protection; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; response to gamma radiation; arachidonic acid metabolic process; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; positive regulation of protein kinase B signaling cascade; response to xenobiotic stimulus; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; heart contraction; cell redox homeostasis; response to lipid hydroperoxide; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress. Disease: Glutathione Peroxidase Deficiency

48-Strip-Wells

410 USD

96-Strip-Wells

490