catalog number :
MBS2516132
products type :
ELISA Kit
products full name :
Human S1P (Sphingosine 1 phosphate) ELISA Kit
products short name :
S1P
other names :
S1P; S1P; CG7169 gene product from transcript CG7169-RA; CG7169-PA; S1P; S1P-PA; S1P
other gene names :
S1P; S1P; CG7169; DmelCG7169; dS1P
uniprot entry name :
Q9VP10_DROME
specificity :
This kit recognizes natural and recombinant Human S1P. No significant cross-reactivity or interference between Human S1P and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Sensitivity: 1.875ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human S1P concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S1P. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S1P and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S1P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of S1P. You can calculate the concentration of S1P in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_649337.2
ncbi gb acc num :
NM_141080.3
ncbi mol weight :
114,305 Da
ncbi pathways :
ATF6-alpha Activates Chaperones Pathway (1116627); Metabolism Pathway (1116326); Metabolism Of Lipids And Lipoproteins Pathway (1116359); Metabolism Of Proteins Pathway (1116564); Protein Processing In Endoplasmic Reticulum Pathway (167200); Protein Processing In Endoplasmic Reticulum Pathway (167190); Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway (1116394); Unfolded Protein Response (UPR) Pathway (1116626)