catalog number :
MBS2516119
products type :
ELISA Kit
products full name :
Human Hepc25 (Hepcidin 25) ELISA Kit
products short name :
[Hepc25]
products gene name :
[Hepc25]
specificity :
This kit recognizes Human HE4 in samples. No Significant cross-reactivity or interference between Human HE4 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50 pmol/mL. Sensitivity: 0.47 pmol/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HE4 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HE4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HE4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HE4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human HE4. You can calculate the concentration of Human HE4 in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells