Rat CD163 (Cluster of Differentiation 163) ELISA Kit | MyBioSource MBS2516101 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat CD163 (Cluster of Differentiation 163) ELISA Kit

catalog :

MBS2516101

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2516101

products type :

ELISA Kit

products full name :

Rat CD163 (Cluster of Differentiation 163) ELISA Kit

products short name :

[CD163]

other names :

[CD163; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen; CD163 molecule; Hemoglobin scavenger receptor; CD_antigen: CD163Soluble CD163; sCD163]

products gene name :

[CD163]

other gene names :

[CD163; CD163; M130; MM130; M130; sCD163]

uniprot entry name :

C163A_HUMAN

reactivity :

Rat

sequence length :

1116

specificity :

This kit recognizes natural and recombinantRatCD163. No significant cross-reactivity or interference between RatCD163 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatCD163concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD163. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD163and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD163, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CD163.You can calculate the concentration of CD163in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

5107945

ncbi acc num :

CAB45233.1

uniprot acc num :

Q86VB7

ncbi mol weight :

124,958 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

ncbi summary :

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: integral to plasma membrane; plasma membrane; extracellular region. Molecular Function: protein binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; acute-phase response

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590