catalog number :
MBS2516097
products type :
ELISA Kit
products full name :
Human HSD17b10 (17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit
products short name :
[HSD17b10]
other names :
[HSD17B10, partial; HSD17B10; hydroxysteroid (17-beta) dehydrogenase 10; HSD17B10]
products gene name :
[HSD17beta10]
other gene names :
[HSD17B10; HSD17B10]
uniprot entry name :
A0A060KPY0_PANTA
specificity :
This kit recognizes natural and recombinant Human HSD17beta10. No significant cross-reactivity or interference between Human HSD17beta10 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 25-1600pg/mL. Sensitivity: Min: 15pg/mL; Max: 1600pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HSD17beta10 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HSD17beta10. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HSD17beta10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSD17beta10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HSD17beta10. You can calculate the concentration of HSD17beta10 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AIC64262.1
ncbi mol weight :
27,280 Da
ncbi pathways :
Alzheimer's Disease Pathway (957117); Alzheimer's Disease Pathway (509); Metabolic Pathways (956960); Valine, Leucine And Isoleucine Degradation Pathway (956929); Valine, Leucine And Isoleucine Degradation Pathway (316)
size5 :
10x96-Strip-Wells