ELISA/assay

Human CCR2 (Chemokine C-C-Motif Receptor 2) ELISA Kit

MBS2516082

24-Strip-Wells

215 USD

ELISA Kit

Human CCR2 (Chemokine C-C-Motif Receptor 2) ELISA Kit

[CCR2]

[C-C chemokine receptor type 2; C-C chemokine receptor type 2; C-C chemokine receptor type 2; MCP-1 receptor; monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor; chemokine (C-C motif) receptor 2; Monocyte chemoattractant protein 1 receptor; MCP-1-R; CD_antigen: CD192]

[CCR2]

[CCR2; CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2; CMKBR2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; MCP-1-R]

CCR2_HUMAN

Human

374

This kit recognizes natural and recombinantHumanCCR2. No significant cross-reactivity or interference between HumanCCR2 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanCCR2concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CCR2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CCR2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CCR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CCR2.You can calculate the concentration of CCR2in the samples by comparing the OD of the samples to the standard curve.

1168965

P41597.1

41,064 Da

Beta Defensins Pathway (530759); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Defensins Pathway (530757); Disease Pathway (530764)

This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009]

CCR2: a seven transmembrane protein closely related to CCR1. Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. Transduces a signal by increasing the intracellular calcium ions level. Alternative co receptor with CD4 for HIV-1 infection. Two splice-variant isoforms have been described. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: cell soma; integral to plasma membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; integral to membrane; plasma membrane; perikaryon; cytosol. Molecular Function: protein homodimerization activity; CCR2 chemokine receptor binding; C-C chemokine receptor activity; chemokine receptor activity. Biological Process: positive regulation of T-helper 1 type immune response; viral reproduction; chemotaxis; elevation of cytosolic calcium ion concentration; response to wounding; dendritic cell chemotaxis; cellular homeostasis; inflammatory response; positive regulation of tumor necrosis factor biosynthetic process; cytokine and chemokine mediated signaling pathway; negative regulation of adenylate cyclase activity; JAK-STAT cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of interferon-gamma production; negative regulation of T-helper 2 type immune response; positive regulation of interleukin-2 production; innate immune response; cellular defense response; blood vessel remodeling; positive regulation of alpha-beta T cell proliferation; immune response; positive regulation of T cell activation; negative regulation of eosinophil degranulation; positive regulation of inflammatory response

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590