catalog number :
MBS2516063
products type :
ELISA Kit
products full name :
Mouse ADM (Adrenomedullin) ELISA Kit
products short name :
[ADM]
other names :
[ADM; ADM; ADM; pro-adrenomedullin; ProAM N-terminal 20 peptide; PAMP; ProAM-N20]
products gene name :
[ADM]
other gene names :
[ADM; ADM; AM; AM; PAMP; ProAM-N20]
uniprot entry name :
ADML_PIG
specificity :
This kit recognizes natural and recombinantMouseADM. No significant cross-reactivity or interference between MouseADM and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseADMconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADMand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADM.You can calculate the concentration of ADMin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_999272.1
ncbi gb acc num :
NM_214107.1
ncbi mol weight :
20,893 Da
ncbi pathways :
Calcitonin-like Ligand Receptors Pathway (1124680); Class B/2 (Secretin Family Receptors) Pathway (1124679); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1124653); Disease Pathway (1124325); G Alpha (s) Signalling Events Pathway (1125293); GPCR Downstream Signaling Pathway (1125292); GPCR Ligand Binding Pathway (1124654); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1124596); Signal Transduction Pathway (1125202); Signaling By GPCR Pathway (1125291)
uniprot summary :
AM and PAMP are potent hypotensive and vasodilatator agents.
size5 :
10x96-Strip-Wells