ELISA/assay

Human OGG1 (8-Oxoguanine Glycosylase 1) ELISA Kit

MBS2516054

24-Strip-Wells

215 USD

ELISA Kit

Human OGG1 (8-Oxoguanine Glycosylase 1) ELISA Kit

[OGG1]

[OGG1 protein type 1b, partial; N-glycosylase/DNA lyase; N-glycosylase/DNA lyase; 8-hydroxyguanine DNA glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; OGG1 type 1f; 8-oxoguanine DNA glycosylase]

[OGG1]

[OGG1; OGG1; HMMH; MUTM; OGH1; HOGG1; MMH; MUTM; OGH1; AP lyase]

OGG1_HUMAN

Human

190

This kit recognizes Human OB in samples. No significant cross-reactivity or interference between Human OB and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.78-50ng/mL. Sensitivity: 0.47ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human OB were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human OB were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human OB concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human OB. During the reaction, Human OB in the sample or standard competes with a fixed amount of Human OB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human OB. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human OB in the samples is then determined by comparing the OD of the samples to the standard curve.

4587158

BAA76639.1

36,085 Da

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Purine Pathway (105842); DNA Repair Pathway (105837); Depurination Pathway (105840); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (105841)

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3 to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: DNA repair, damage; Deoxyribonuclease; EC 4.2.99.18; Lyase. Chromosomal Location of Human Ortholog: 3p26.2. Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck. Molecular Function: protein binding; microtubule binding; endonuclease activity; DNA N-glycosylase activity; oxidized purine base lesion DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity. Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; regulation of transcription, DNA-dependent; base-excision repair, AP site formation; nucleotide-excision repair; base-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; negative regulation of apoptosis; aging. Disease: Renal Cell Carcinoma, Nonpapillary

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590