ELISA/assay

Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

MBS2516043

24-Strip-Wells

215 USD

ELISA Kit

Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

[IGFBP5]

[insulin-like growth factor-binding protein 5; Insulin-like growth factor-binding protein 5; insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; insulin-like growth factor binding protein 5]

[IGFBP5]

[IGFBP5; IGFBP5; IBP5; IBP5; IBP-5; IGF-binding protein 5; IGFBP-5]

IBP5_HUMAN

Mouse

272

This kit recognizes natural and recombinantMouseIGFBP5. No significant cross-reactivity or interference between MouseIGFBP5 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 125-8000pg/mL. Sensitivity: Min: 75pg/mL; Max: 8000pg/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseIGFBP5concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGFBP5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IGFBP5and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGFBP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IGFBP5.You can calculate the concentration of IGFBP5in the samples by comparing the OD of the samples to the standard curve.

10834982

NP_000590.1

NM_000599.3

30,570 Da

Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908); Senescence And Autophagy Pathway (198780)

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region. Molecular Function: protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding. Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; signal transduction; glucose homeostasis; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; skeletal muscle growth; regulation of cell growth; negative regulation of cell migration

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590