ELISA/assay

Human IL-25 (Interleukin 25) ELISA Kit

MBS2516038

24-Strip-Wells

215 USD

ELISA Kit

Human IL-25 (Interleukin 25) ELISA Kit

[IL-25]

[interleukin-25 isoform 2; Interleukin-25; interleukin-25; interleukin-17E; interleukin 25; Interleukin-17E; IL-17E]

[IL-25]

[IL25; IL25; IL17E; IL17E; IL-25; IL-17E]

IL25_HUMAN

Human

161

This kit recognizes Human IL-12 in samples. No significant cross-reactivity or interference between Human IL-12 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-12 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-12 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-12 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-12. You can calculate the concentration of Human IL-12 in the samples by comparing the OD of the samples to the standard curve.

27477081

NP_758525.1

NM_172314.1

18,523 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968)

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular space. Molecular Function: cytokine activity; interleukin-17E receptor binding. Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590