catalog number :
MBS2516027
products type :
ELISA Kit
products full name :
Mouse CD8 (Cluster of Differentiation 8) ELISA Kit
products short name :
[CD8]
other names :
[CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide; CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); T-cell surface molecule]
products gene name :
[CD8]
other gene names :
[CD8A; CD8]
specificity :
This kit recognizes natural and recombinantMouseCD8. No significant cross-reactivity or interference between MouseCD8 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.25-80ng/mL. Sensitivity: Min: 0.75ng/mL; Max: 80ng/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseCD8concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD8. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD8and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CD8.You can calculate the concentration of CD8in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_990566.1
ncbi gb acc num :
NM_205235.1
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (119314); Cell Adhesion Molecules (CAMs) Pathway (480)
size5 :
10x96-Strip-Wells