Mouse UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit | MyBioSource MBS2516002 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

catalog :

MBS2516002

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2516002

products type :

ELISA Kit

products full name :

Mouse UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

products short name :

UCHL1

other names :

ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase isozyme L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin thioesterase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1

products gene name :

UCHL1

other gene names :

UCHL1; UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; UCH-L1; PGP9.5

uniprot entry name :

UCHL1_HUMAN

reactivity :

Mouse

sequence length :

223

specificity :

This kit recognizes natural and recombinant Mouse UCHL1. No significant cross-reactivity or interference between Mouse UCHL1 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse UCHL1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UCHL1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of UCHL1. You can calculate the concentration of UCHL1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

21361091

ncbi acc num :

NP_004172.2

ncbi gb acc num :

NM_004181.4

ncbi mol weight :

24,824 Da

ncbi pathways :

Alpha-synuclein Signaling Pathway (137913); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Proteasome Degradation Pathway (198786)

ncbi summary :

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

uniprot summary :

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family. Protein type: Ligase; EC 3.4.19.12; Protease; Ubiquitin conjugating system; Cell development/differentiation. Chromosomal Location of Human Ortholog: 4p14. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol. Molecular Function: protein binding; ubiquitin binding; omega peptidase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity. Biological Process: cell proliferation; negative regulation of MAP kinase activity; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; muscle fiber development; sensory perception of pain; adult walking behavior. Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage