Human CPT1A (Carnitine Palmitoyltransferase 1A, Liver) ELISA Kit | MyBioSource MBS2515998 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CPT1A (Carnitine Palmitoyltransferase 1A, Liver) ELISA Kit

catalog :

MBS2515998

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515998

products type :

ELISA Kit

products full name :

Human CPT1A (Carnitine Palmitoyltransferase 1A, Liver) ELISA Kit

products short name :

[CPT1A]

other names :

[carnitine O-palmitoyltransferase 1, liver isoform isoform 1; Carnitine O-palmitoyltransferase 1, liver isoform; carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPTI-L; carnitine O-palmitoyltransferase I, liver isoform; carnitine palmitoyltransferase I, liver; carnitine palmitoyltransferase 1A (liver); Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A]

products gene name :

[CPT1A]

other gene names :

[CPT1A; CPT1A; CPT1; CPT1-L; L-CPT1; CPT1; CPT1-L; CPT I; CPTI-L]

uniprot entry name :

CPT1A_HUMAN

reactivity :

Human

sequence length :

773

specificity :

This kit recognizes Human CoQ10B in samples. No significant cross-reactivity or interference between Human CoQ10B and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.63-40ng/mL. Sensitivity: 0.38ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CoQ10B were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CoQ10B were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CoQ10B concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CoQ10B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CoQ10B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CoQ10B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CoQ10B. You can calculate the concentration of Human CoQ10B in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

73623030

ncbi acc num :

NP_001867.2

ncbi gb acc num :

NM_001876.3

ncbi mol weight :

86,239 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Fatty Acid Beta Oxidation Pathway (198865); Fatty Acid Degradation Pathway (82935); Fatty Acid Degradation Pathway (296)

ncbi summary :

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; EC 2.3.1.21; Mitochondrial; Membrane protein, integral; Transferase; Lipid Metabolism - fatty acid. Chromosomal Location of Human Ortholog: 11q13.2. Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; mitochondrial inner membrane; integral to mitochondrial outer membrane. Molecular Function: identical protein binding; carnitine O-palmitoyltransferase activity. Biological Process: response to drug; circadian rhythm; eating behavior; glucose metabolic process; carnitine shuttle; long-chain fatty acid metabolic process; cellular lipid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; fatty acid beta-oxidation; triacylglycerol metabolic process; epithelial cell differentiation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization. Disease: Carnitine Palmitoyltransferase I Deficiency

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590