ELISA/assay

Canine CKM (Creatine Kinase, Muscle) ELISA Kit

MBS2515957

24-Strip-Wells

255 USD

ELISA Kit

Canine CKM (Creatine Kinase, Muscle) ELISA Kit

[CKM]

[Creatine kinase M-type; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CKCreatine kinase M-type, N-terminally processed]

[CKM]

[CKM; CKM; CKMM; M-CK; CKMM]

KCRM_HUMAN

Canine

381

This kit recognizes beta-actin in samples. No significant cross-reactivity or interference between beta-actin and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.16-10ng/mL. Sensitivity: 0.09ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level beta-actin were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level beta-actin were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of beta-actin concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with beta-actin. During the reaction, beta-actin in the sample or standard competes with a fixed amount of beta-actin on the solid phase supporter for sites on the Biotinylated Detection Ab specific to beta-actin. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of beta-actin in the samples is then determined by comparing the OD of the samples to the standard curve.

125305

P06732.2

43,101 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Retinoblastoma Protein Pathway (137916); Urea Cycle And Metabolism Of Amino Groups Pathway (198758)

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: cytosol. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; phosphorylation; creatine metabolic process

24-Strip-Wells

255 USD

48-Strip-Wells

490

96-Strip-Wells

585

5x96-Strip-Wells

2475

10x96-Strip-Wells

4360