catalog number :
MBS2515937
products type :
ELISA Kit
products full name :
Rat EGFR (Epidermal Growth Factor Receptor) ELISA Kit
products short name :
[EGFR]
other names :
[epidermal growth factor receptor, partial; Epidermal growth factor receptor; Epidermal growth factor receptor]
products gene name :
[EGFR]
other gene names :
[c-erbB proto-oncogene]
uniprot entry name :
Q9P2Z9_HUMAN
specificity :
This kit recognizes natural and recombinant Rat EGFR. No significant cross-reactivity or interference between Rat EGFR and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat EGFR concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EGFR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EGFR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EGFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EGFR. You can calculate the concentration of EGFR in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB19486.2
ncbi mol weight :
1,020 Da
size5 :
10x96-Strip-Wells