catalog number :
MBS2515930
products type :
ELISA Kit
products full name :
Human PTP1B(protein tyrosine phosphatase 1B)ELISA Kit
products short name :
PTP1B
other names :
Ptp1b protein; Tyrosine-protein phosphatase non-receptor type; tyrosine-protein phosphatase non-receptor type 1; PTP-1B; fk54h03; protein tyrosine phosphatase 1b; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1
products gene name :
PTP1B
other gene names :
ptpn1; ptpn1; ptp1b; wu:fk54h03
uniprot entry name :
Q7SYN6_DANRE
specificity :
This assay has high sensitivity and excellent specificity for detection of Human PTP1B. No significant cross-reactivity or interference between Human PTP1B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PTP1B and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125--5000pg/mL. Sensitivity: 46.875pg/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PTP1B in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTP1B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PTP1B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTP1B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PTP1B. You can calculate the concentration of Human PTP1B in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAI64734.1
ncbi mol weight :
49,124 Da
ncbi pathways :
Adherens Junction Pathway (119268); Adherens Junction Pathway (481); Cytokine Signaling In Immune System Pathway (1114805); ERK1 - ERK2 MAPK Cascade Pathway (198962); FGF Signaling Pathway (198948); Growth Hormone Receptor Signaling Pathway (1114822); Hemostasis Pathway (1114916); Immune System Pathway (1114705); Insulin Signaling Pathway (921015); Insulin Signaling Pathway (119273)