catalog number :
MBS2515913
products type :
ELISA Kit
products full name :
Mouse SDC1 (Syndecan 1) ELISA Kit
products short name :
[SDC1]
other names :
[syndecan-1; Syndecan-1; syndecan-1; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan proteoglycan 1; syndecan 1; CD_antigen: CD138]
products gene name :
[SDC1]
other gene names :
[SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1]
uniprot entry name :
SDC1_HUMAN
specificity :
This kit recognizes Mouse SAA in samples. No significant cross-reactivity or interference between Mouse SAA and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse SAA were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse SAA were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse SAA concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SAA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SAA. You can calculate the concentration of Mouse SAA in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001006947.1
ncbi gb acc num :
NM_001006946.1
ncbi mol weight :
32,462 Da
ncbi pathways :
A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Arf6 Trafficking Events Pathway (137954); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Chylomicron-mediated Lipid Transport Pathway (106157); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591)
ncbi summary :
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
uniprot summary :
syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family. Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: lysosomal lumen; cell surface; protein complex; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; myoblast development; response to cAMP; glycosaminoglycan metabolic process; wound healing; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; pathogenesis; lipoprotein metabolic process; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; odontogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; response to calcium ion; inflammatory response; retinoid metabolic process
size5 :
10x96-Strip-Wells