catalog number :
MBS2515900
products type :
ELISA Kit
products full name :
Human CFHR3 (Complement Factor H Related Protein 3) ELISA Kit
products short name :
[CFHR3]
other names :
[complement factor H-related protein 3 isoform 2; Complement factor H-related protein 3; complement factor H-related protein 3; H factor-like 4; H factor-like protein 3; complement factor H-related 3; DOWN16; H factor-like protein 3]
products gene name :
[CFHR3]
other gene names :
[CFHR3; CFHR3; FHR3; HLF4; CFHL3; FHR-3; DOWN16; CFHL3; FHR3; FHR-3]
uniprot entry name :
FHR3_HUMAN
specificity :
This kit recognizes natural and recombinantHumanCFHR3. No significant cross-reactivity or interference between HumanCFHR3 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanCFHR3concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFHR3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFHR3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFHR3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CFHR3.You can calculate the concentration of CFHR3in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001160096.1
ncbi gb acc num :
NM_001166624.1
ncbi mol weight :
30,720 Da
ncbi pathways :
Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Regulation Of Complement Cascade Pathway (576254)
ncbi summary :
The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
uniprot summary :
CFHR3: Might be involved in complement regulation. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space. Disease: Macular Degeneration, Age-related, 1; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 1
size5 :
10x96-Strip-Wells