catalog number :
MBS2515885
products type :
ELISA Kit
products full name :
Mouse PIBF(progesterone induced blocking factor)ELISA Kit
products short name :
[PIBF]
other names :
[progesterone-induced-blocking factor 1; Progesterone-induced-blocking factor 1; progesterone-induced-blocking factor 1; progesterone-induced blocking factor 1; progesterone immunomodulatory binding factor 1]
products gene name :
[PIBF]
other gene names :
[PIBF1; PIBF1; PIBF; CEP90; C13orf24; C13orf24; PIBF]
uniprot entry name :
PIBF1_HUMAN
specificity :
This kit recognizes Mouse PEDF in samples. No significant cross-reactivity or interference between Mouse PEDF and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PEDF were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PEDF were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PEDF concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PEDF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PEDF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PEDF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PEDF. You can calculate the concentration of Mouse PEDF in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_006337.2
ncbi gb acc num :
NM_006346.2
ncbi mol weight :
12,016 Da
uniprot summary :
PIBF1: Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. 3 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 13q22.1. Cellular Component: centrosome. Molecular Function: protein binding
size5 :
10x96-Strip-Wells