ELISA/assay

Mouse ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit

MBS2515880

24-Strip-Wells

215 USD

ELISA Kit

Mouse ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit

[ALCAM]

[alcam; CD166 antigen; CD166 antigen; activated leukocyte cell adhesion molecule; Activated leukocyte cell adhesion molecule; CD_antigen: CD166]

[ALCAM]

[ALCAM; ALCAM; MEMD; CD166; MEMD]

CD166_HUMAN

Mouse

583

This kit recognizes natural and recombinantMouseALCAM. No significant cross-reactivity or interference between MouseALCAM and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseALCAMconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ALCAM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ALCAMand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ALCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ALCAM.You can calculate the concentration of ALCAMin the samples by comparing the OD of the samples to the standard curve.

886258

AAB59499.1

33,878 Da

Axon Guidance Pathway (105688); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (477129); L1CAM Interactions Pathway (161003)

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 3q13.1. Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; receptor binding. Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590