catalog number :
MBS2515860
products type :
ELISA Kit
products full name :
Bb(Bilirubin) ELISA Kit
products short name :
[Bb]
products gene name :
[Bb]
specificity :
This kit recognizes natural and recombinantBb. No significant cross-reactivity or interference between Bb and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Competitive. Detection Range: 0.313-20?g/mL. Sensitivity: Min: 0.188?g/mL; Max: 20?g/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of Bbconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Bb. During the reaction,Bb in the sample or standard competes with a fixed amount ofBb on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bb. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration ofBb in the samples is then determined by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells