Rat AQP-2 (Aquaporin 2) ELISA Kit | MyBioSource MBS2515840 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat AQP-2 (Aquaporin 2) ELISA Kit

catalog :

MBS2515840

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515840

products type :

ELISA Kit

products full name :

Rat AQP-2 (Aquaporin 2) ELISA Kit

products short name :

[AQP-2]

other names :

[aquaporin-2; Aquaporin-2; aquaporin-2; ADH water channel; AQP-2; aquaporin-CD; collecting duct water channel protein; water channel protein for renal collecting duct; water-channel aquaporin 2; aquaporin 2 (collecting duct); ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct]

products gene name :

[AQP-2]

other gene names :

[AQP2; AQP2; AQP-CD; WCH-CD; AQP-2; AQP-CD]

uniprot entry name :

AQP2_HUMAN

reactivity :

Rat

sequence length :

271

specificity :

This kit recognizes natural and recombinant Rat AQP-2. No significant cross-reactivity or interference between Rat AQP-2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat AQP-2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AQP-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AQP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AQP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AQP-2. You can calculate the concentration of AQP-2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4502179

ncbi acc num :

NP_000477.1

ncbi gb acc num :

NM_000486.5

ncbi mol weight :

28,837 Da

ncbi pathways :

Aquaporin-mediated Transport Pathway (187187); Passive Transport By Aquaporins Pathway (187188); Transmembrane Transport Of Small Molecules Pathway (106572); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (187189); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)

ncbi summary :

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

uniprot summary :

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family. Protein type: Transporter; Transporter, aquaporin family; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc. Chromosomal Location of Human Ortholog: 12q12-q13. Cellular Component: recycling endosome; protein complex; membrane; rough endoplasmic reticulum; clathrin-coated vesicle; basolateral plasma membrane; integral to plasma membrane; lysosome; early endosome; apical plasma membrane; plasma membrane; trans-Golgi network. Molecular Function: water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; glycerol channel activity; PDZ domain binding. Biological Process: glycerol transport; actin filament depolymerization; cellular water homeostasis; response to glucagon stimulus; apoptosis; water transport; response to lithium ion; cellular response to water deprivation; response to lipopolysaccharide; female pregnancy; excretion; response to salt stress; response to starvation; hyperosmotic response; renal water transport; renal water homeostasis; response to calcium ion; positive regulation of calcium ion transport; transmembrane transport; aging. Disease: Diabetes Insipidus, Nephrogenic, Autosomal

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590