Rat MMP-14 (Matrix Metalloproteinase 14) ELISA Kit | MyBioSource MBS2515839 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat MMP-14 (Matrix Metalloproteinase 14) ELISA Kit

catalog :

MBS2515839

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515839

products type :

ELISA Kit

products full name :

Rat MMP-14 (Matrix Metalloproteinase 14) ELISA Kit

products short name :

[MMP-14]

other names :

[matrix metalloproteinase-14 preproprotein; Matrix metalloproteinase-14; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase; matrix metallopeptidase 14 (membrane-inserted); MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP]

products gene name :

[MMP-14]

other gene names :

[MMP14; MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP]

uniprot entry name :

MMP14_HUMAN

reactivity :

Rat

sequence length :

582

specificity :

This kit recognizes natural and some recombinant Rat MMP-14. No significant crossreactivity or interference between Rat MMP-14 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat MMP-14 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat MMP-14. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat MMP-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat MMP-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-14. The concentration of Rat MMP-14 in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

4826834

ncbi acc num :

NP_004986.1

ncbi gb acc num :

NM_004995.3

ncbi mol weight :

65,894 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); GnRH Signaling Pathway (83091); GnRH Signaling Pathway (502); HIF-2-alpha Transcription Factor Network Pathway (137956); Matrix Metalloproteinases Pathway (198900); Senescence And Autophagy Pathway (198780)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family. Protein type: Motility/polarity/chemotaxis; Protease; Membrane protein, integral; EC 3.4.24.80. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; plasma membrane; melanosome. Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity. Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; male gonad development; negative regulation of focal adhesion formation; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; extracellular matrix disassembly; collagen catabolic process; branching morphogenesis of a tube; regulation of transcription, DNA-dependent; ovarian follicle development; response to mechanical stimulus; response to estrogen stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; angiogenesis; response to oxidative stress; astrocyte cell migration; endochondral ossification; positive regulation of cell migration; lung development. Disease: Winchester Syndrome

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590