Human IL4I1 (Interleukin 4 Induced Protein 1) ELISA Kit | MyBioSource MBS2515832 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human IL4I1 (Interleukin 4 Induced Protein 1) ELISA Kit

catalog :

MBS2515832

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2515832

products type :

ELISA Kit

products full name :

Human IL4I1 (Interleukin 4 Induced Protein 1) ELISA Kit

products short name :

[IL4I1]

other names :

[interleukin-4 induced protein-1 variant 2; L-amino-acid oxidase; L-amino-acid oxidase; Fig-1 protein; IL4-induced protein 1; interleukin four induced 1; interleukin 4 induced 1; Interleukin-4-induced protein 1; IL4-induced protein 1; Protein Fig-1; hFIG1]

products gene name :

[IL4I1]

other gene names :

[IL4I1; IL4I1; LAO; FIG1; LAAO; FIG1; LAAO; LAO; IL4-induced protein 1; hFIG1]

uniprot entry name :

OXLA_HUMAN

reactivity :

Human

sequence length :

589

specificity :

This kit recognizes Human IL-35 in samples. No significant cross-reactivity or interference between Human IL-35 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-35 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-35 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-35. You can calculate the concentration of Human IL-35 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

71483573

ncbi acc num :

AAZ32713.1

ncbi mol weight :

65,328 Da

ncbi pathways :

Abnormal Metabolism In Phenylketonuria Pathway (645287); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Disease Pathway (530764); Metabolic Pathways (132956); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Phenylalanine, Tyrosine And Tryptophan Biosynthesis Pathway (82965)

ncbi summary :

This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

IL4I1: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation. Belongs to the flavin monoamine oxidase family. FIG1 subfamily. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - cysteine and methionine; Secreted; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Secreted, signal peptide; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - alanine, aspartate and glutamate; Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.4.3.2. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: lysosome; extracellular region. Molecular Function: L-amino-acid oxidase activity

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage