Rat FLAP (5-Lipoxygenase Activating Protein) ELISA Kit | MyBioSource MBS2515825 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat FLAP (5-Lipoxygenase Activating Protein) ELISA Kit

catalog :

MBS2515825

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515825

products type :

ELISA Kit

products full name :

Rat FLAP (5-Lipoxygenase Activating Protein) ELISA Kit

products short name :

[FLAP]

other names :

[FLAP; Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein]

products gene name :

[FLAP]

other gene names :

[ALOX5AP; FLAP]

uniprot entry name :

AL5AP_HUMAN

reactivity :

Rat

sequence length :

161

specificity :

This kit recognizes Rat FGF23 in samples. No significant cross-reactivity or interference between Rat FGF23 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat FGF23 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat FGF23 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat FGF23 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat FGF23. You can calculate the concentration of Rat FGF23 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

355754608

ncbi acc num :

EHH58509.1

uniprot acc num :

P20292

ncbi mol weight :

18,157 Da

uniprot summary :

ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family. Protein type: Membrane protein, multi-pass; Nuclear envelope; Activator; Membrane protein, integral; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope. Molecular Function: arachidonic acid binding; protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; protein N-terminus binding; glutathione peroxidase activity. Biological Process: positive regulation of catalytic activity; leukotriene biosynthetic process; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process. Disease: Stroke, Ischemic

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590