catalog number :
MBS2515814
products type :
ELISA Kit
products full name :
Human ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit
products short name :
[ANGPTL8]
other names :
[angiopoietin-like protein 8; Angiopoietin-like protein 8; angiopoietin-like protein 8; betatrophin variant 1; betatrophin variant 2; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; lipasin; refeeding-induced fat and liver protein; chromosome 19 open reading frame 80; Betatrophin; Lipasin; Refeeding-induced fat and liver protein]
products gene name :
[ANGPTL8]
other gene names :
[C19orf80; C19orf80; RIFL; TD26; ANGPTL8; PRO1185; PVPA599]
uniprot entry name :
BETAT_HUMAN
specificity :
This kit recognizes natural and some recombinant Human ANGPTL8. No significant crossreactivity or interference between Human ANGPTL8 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 125-8000pg/mL. Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human ANGPTL8 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human ANGPTL8. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human ANGPTL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human ANGPTL8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ANGPTL8. The concentration of Human ANGPTL8 in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
NP_061157.3
ncbi gb acc num :
NM_018687.6
ncbi mol weight :
22,105 Da
uniprot summary :
EG624219: Hormone that specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance. Promotes pancreatic beta cell proliferation without insulin resistance. Also acts as a blood lipid regulator by regulating serum triglyceride levels; possibly by promoting ANGPTL3 cleavage. In response to food intake. Interacts with ANGPTL3. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular region. Molecular Function: protein binding; hormone activity. Biological Process: fat cell differentiation; regulation of lipid metabolic process; cell maturation; regulation of lipoprotein metabolic process; cellular lipid metabolic process
size5 :
10x96-Strip-Wells