catalog number :
MBS2515780
products type :
ELISA Kit
products full name :
Human IL31(Interleukin 31)ELISA Kit
products short name :
[IL31]
other names :
[interleukin-31; Interleukin-31; interleukin-31; interleukin 31]
products gene name :
[IL31]
other gene names :
[IL31; IL31; IL-31; IL-31]
uniprot entry name :
IL31_HUMAN
specificity :
This kit recognizes Human IL-25 in samples. No Significant cross-reactivity or interference between Human IL-25 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000 pg/mL. Sensitivity: 18.75 pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-25 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-25 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-25 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-25. You can calculate the concentration of Human IL-25 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001014358.1
ncbi gb acc num :
NM_001014336.1
ncbi mol weight :
18,205 Da
ncbi summary :
IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]
uniprot summary :
IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 12q24.31. Cellular Component: extracellular space. Molecular Function: cytokine activity. Biological Process: immune system process
size5 :
10x96-Strip-Wells