ELISA/assay

Human HPF4(Heparin/Platelet Factor 4 Antibodies)ELISA Kit

MBS2515736

48-Strip-Wells

410 USD

ELISA Kit

Human HPF4(Heparin/Platelet Factor 4 Antibodies)ELISA Kit

HPF4

HPF4

Human

This kit recognizes natural and recombinant Human HPF4. No significant cross-reactivity or interference between Human HPF4 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: The minimum detectable dose of Human HPF4 is 0.375ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HPF4 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HPF4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HPF4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HPF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HPF4. You can calculate the concentration of HPF4 in the samples by comparing the OD of the samples to the standard curve.

48-Strip-Wells

410 USD

96-Strip-Wells

490