Rat NPHN (Nephrin) ELISA Kit | MyBioSource MBS2515727 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat NPHN (Nephrin) ELISA Kit

catalog :

MBS2515727

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515727

products type :

ELISA Kit

products full name :

Rat NPHN (Nephrin) ELISA Kit

products short name :

[NPHN]

other names :

[nephrin; Nephrin; nephrin; renal glomerulus-specific cell adhesion receptor; nephrosis 1, congenital, Finnish type (nephrin); Renal glomerulus-specific cell adhesion receptor]

products gene name :

[NPHN]

other gene names :

[NPHS1; NPHS1; CNF; NPHN; nephrin; NPHN]

uniprot entry name :

NPHN_HUMAN

reactivity :

Rat

sequence length :

1241

specificity :

This kit recognizes natural and recombinant Rat NPHN. No significant cross-reactivity or interference between Rat NPHN and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NPHN concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NPHN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NPHN. You can calculate the concentration of NPHN in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4758822

ncbi acc num :

NP_004637.1

ncbi gb acc num :

NM_004646.3

ncbi mol weight :

130,546 Da

ncbi pathways :

Cell-Cell Communication Pathway (477132); Nephrin Interactions Pathway (187228); Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway (138072)

ncbi summary :

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

uniprot summary :

NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Immunoglobulin superfamily; Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cell projection; protein complex; integral to plasma membrane; plasma membrane; lipid raft. Molecular Function: protein domain specific binding; protein binding; myosin binding; spectrin binding; alpha-actinin binding. Biological Process: skeletal muscle development; positive regulation of actin filament polymerization; glomerular basement membrane development; JNK cascade; cell adhesion; excretion; myoblast fusion. Disease: Nephrotic Syndrome, Type 1

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590