catalog number :
MBS2515724
products type :
ELISA Kit
products full name :
Human VEGF (Vascular Endothelial Cell Growth Factor) ELISA Kit
products short name :
[VEGF]
other names :
[VEGF, partial; Vascular endothelial growth factor A; vascular endothelial growth factor A; VEGF-A; VPF; vascular permeability factor; Vascular permeability factor; VPF]
products gene name :
[VEGF]
other gene names :
[VEGFA; VEGFA; VEGF; VEGF; VEGF-A; VPF]
uniprot entry name :
VEGFA_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Human VEGF. No significant cross-reactivity or interference between Human VEGF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human VEGF and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25--2000pg/mL. Sensitivity: 18.75pg/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGF in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human VEGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human VEGF. You can calculate the concentration of Human VEGF in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AIE11421.1
ncbi mol weight :
22,368 Da
ncbi pathways :
Axon Guidance Pathway (1124275); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (1125445); Cellular Responses To Stress Pathway (1125444); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1124274); EPH-Ephrin Signaling Pathway (1124309); EPHA-mediated Growth Cone Collapse Pathway (1124310)
uniprot summary :
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin ().
size5 :
10x96-Strip-Wells