ELISA/assay

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit

MBS2515711

24-Strip-Wells

215 USD

ELISA Kit

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit

[IL-12 p35]

[interleukin-12 subunit alpha; Interleukin-12 subunit alpha; interleukin-12 subunit alpha; CLMF p35; IL-12, subunit p35; IL35 subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin 12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1]

[IL-12 p35]

[IL12A; IL12A; P35; CLMF; NFSK; NKSF1; IL-12A; NKSF1; IL-12A; CLMF p35; NKSF1]

IL12A_HUMAN

Human

253

This kit recognizes natural and recombinant Human IL-12 p35. No significant cross-reactivity or interference between Human IL-12 p35 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/mL. Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-12 p35 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-12 p35. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-12 p35 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-12 p35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-12 p35. You can calculate the concentration of IL-12 p35 in the samples by comparing the OD of the samples to the standard curve. FOR

24430219

NP_000873.2

NM_000882.3

24,874 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (83051)

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q25.33. Cellular Component: extracellular space; interleukin-12 complex; cytoplasm. Molecular Function: interleukin-27 binding; protein binding; interleukin-12 beta subunit binding; growth factor activity; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding. Biological Process: cell migration; positive regulation of NK T cell activation; positive regulation of cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; negative regulation of smooth muscle cell proliferation; positive regulation of T cell mediated cytotoxicity; response to virus; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of natural killer cell mediated cytotoxicity; defense response to protozoan; positive regulation of natural killer cell activation; response to UV-B; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of mononuclear cell proliferation; positive regulation of T cell differentiation; positive regulation of lymphocyte proliferation; positive regulation of T cell proliferation; immune response; cell cycle arrest

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590