ELISA/assay

Human DNMT1(DNA Methyltransferase 1) ELISA Kit

MBS2515666

24-Strip-Wells

215 USD

ELISA Kit

Human DNMT1(DNA Methyltransferase 1) ELISA Kit

[DNMT1]

[DNA (cytosine-5)-methyltransferase 1 isoform a; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA MTase HsaI; DNA methyltransferase HsaI; m.HsaI; DNA (cytosine-5-)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT]

[DNMT1]

[DNMT1; DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI]

DNMT1_HUMAN

Human

1632

This kit recognizes Human DKK2 in samples. No Significant cross-reactivity or interference between Human DKK2 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.10 ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human DKK2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human DKK2 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human DKK2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DKK2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DKK2. You can calculate the concentration of Human DKK2 in the samples by comparing the OD of the samples to the standard curve.

195927037

NP_001124295.1

NM_001130823.1

144,465 Da

Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); DNA Methylation Pathway (1127677); Epigenetic Regulation Of Gene Expression Pathway (1127671); Gene Expression Pathway (105937); Metabolic Pathways (132956); Methionine Degradation Pathway (413353); Methionine Degradation Pathway (468228); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928)

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described. Protein type: Cell development/differentiation; Methyltransferase; Methyltransferase, DNA; EC 2.1.1.37; Amino Acid Metabolism - cysteine and methionine; Transcription regulation. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus. Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; methyl-CpG binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding. Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of transcription from RNA polymerase II promoter; gene silencing; positive regulation of histone H3-K4 methylation; chromatin modification; regulation of gene expression, epigenetic; regulation of cell proliferation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; DNA methylation on cytosine. Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590