Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit | MyBioSource MBS2515630 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit

catalog :

MBS2515630

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515630

products type :

ELISA Kit

products full name :

Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit

products short name :

[DKK3]

other names :

[dickkopf-related protein 3; Dickkopf-related protein 3; dickkopf-related protein 3; RIG-like 5-6; RIG-like 7-1; dickkopf 3 homolog; dickkopf homolog 3; dickkopf-3; dkk-3; hDkk-3; regulated in glioma; dickkopf WNT signaling pathway inhibitor 3]

products gene name :

[DKK3]

other gene names :

[DKK3; DKK3; RIG; REIC; REIC; Dickkopf-3; Dkk-3; hDkk-3]

uniprot entry name :

DKK3_HUMAN

reactivity :

Mouse

sequence length :

350

specificity :

This kit recognizes Mouse cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Mouse cTnT/TNNT2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse cTnT/TNNT2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse cTnT/TNNT2 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse cTnT/TNNT2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse cTnT/TNNT2. You can calculate the concentration of Mouse cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

66346689

ncbi acc num :

NP_001018067.1

ncbi gb acc num :

NM_001018057.1

ncbi mol weight :

38,390 Da

ncbi summary :

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: extracellular space. Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590