Human 5-NT (5-Nucleotidase) ELISA Kit | MyBioSource MBS2515616 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 5-NT (5-Nucleotidase) ELISA Kit

catalog :

MBS2515616

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515616

products type :

ELISA Kit

products full name :

Human 5-NT (5-Nucleotidase) ELISA Kit

products short name :

[5-NT]

other names :

[5'-nucleotidase isoform 2 preproprotein; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; 5'-nucleotidase, ecto (CD73); Ecto-5'-nucleotidase; CD_antigen: CD73]

products gene name :

[5-NT]

other gene names :

[NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; NT5; NTE; 5'-NT]

uniprot entry name :

5NTD_HUMAN

reactivity :

Human

sequence length :

524

specificity :

This assay has high sensitivity and excellent specificity for detection of Human 5-NT. No significant cross-reactivity or interference between Human 5-NT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human 5-NT and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of 5-NT in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human 5-NT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-NT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human 5-NT. You can calculate the concentration of Human 5-NT in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

325651886

ncbi acc num :

NP_001191742.1

ncbi gb acc num :

NM_001204813.1

uniprot acc num :

P21589

ncbi mol weight :

57,949 Da

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (106276); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307)

ncbi summary :

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

uniprot summary :

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 3.1.3.5; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, GPI anchor; Phosphatase (non-protein); Membrane protein, integral. Chromosomal Location of Human Ortholog: 6q14-q21. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; nucleotide binding. Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process. Disease: Calcification Of Joints And Arteries

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590