ELISA/assay

Human MYO (Myoglobin) ELISA Kit

MBS2515596

24-Strip-Wells

190 USD

ELISA Kit

Human MYO (Myoglobin) ELISA Kit

[MYO]

[myogenin; Myogenin; myogenin; MYOD1-related protein; myogenin; MYOD1-related protein]

[MYO]

[Myog; Myog; myo; MYF4; bHLHc3]

MYOG_MOUSE

Human

224

This kit recognizes natural and some recombinant Human MYO. No significant cross-reactivity or interference between Human MYO and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human MYO concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human MYO and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MYO. The concentration of Human MYO in samples can be calculated by comparing the OD of the samples with the standard curve.

13654247

NP_112466.1

NM_031189.2

P12979

25,203 Da

Hypertrophy Model Pathway (198326); Id Signaling Pathway (198411)

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. Protein type: DNA-binding; Transcription factor. Cellular Component: nucleus. Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; muscle development; multicellular organismal development; positive regulation of transcription, DNA-dependent; skeletal muscle fiber development; response to denervation involved in regulation of muscle adaptation; negative regulation of cell proliferation; positive regulation of skeletal muscle fiber development; regulation of transcription, DNA-dependent; cell differentiation; negative regulation of glycolysis; skeletal muscle development; ossification; transcription, DNA-dependent; myotube differentiation; response to muscle activity involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; cell cycle; mRNA transcription from RNA polymerase II promoter; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; striated muscle atrophy; positive regulation of muscle atrophy; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation

24-Strip-Wells

190 USD

48-Strip-Wells

310

96-Strip-Wells

355

5x96-Strip-Wells

1435

10x96-Strip-Wells

2805