Human PDXK (Pyridoxal Kinase) ELISA Kit | MyBioSource MBS2515526 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PDXK (Pyridoxal Kinase) ELISA Kit

catalog :

MBS2515526

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2515526

products type :

ELISA Kit

products full name :

Human PDXK (Pyridoxal Kinase) ELISA Kit

products short name :

[PDXK]

other names :

[PDXK protein; Pyridoxal kinase; pyridoxal kinase; epididymis secretory sperm binding protein Li 1a; pyridoxamine kinase; pyridoxine kinase; vitamin B6 kinase; pyridoxal (pyridoxine, vitamin B6) kinase; Pyridoxine kinase]

products gene name :

[PDXK]

other gene names :

[PDXK; PDXK; PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124; C21orf124; C21orf97; PKH; PNK]

uniprot entry name :

PDXK_HUMAN

reactivity :

Human

sequence length :

284

specificity :

This kit recognizes Human PDPN in samples. No significant cross-reactivity or interference between Human PDPN and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PDPN were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PDPN were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PDPN concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDPN. You can calculate the concentration of Human PDPN in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

13543317

ncbi acc num :

AAH05825.1

uniprot acc num :

O00764

ncbi mol weight :

26,827 Da

ncbi pathways :

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

ncbi summary :

The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.35; Cofactor and Vitamin Metabolism - vitamin B6; Kinase, other. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus. Molecular Function: potassium ion binding; sodium ion binding; protein homodimerization activity; zinc ion binding; pyridoxal kinase activity; magnesium ion binding; lithium ion binding; ATP binding; pyridoxal phosphate binding. Biological Process: cell proliferation; vitamin metabolic process; vitamin B6 metabolic process; pyridoxal 5 -phosphate salvage; phosphorylation; water-soluble vitamin metabolic process; pyridoxal phosphate biosynthetic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590