Porcine VCAM-1/CD106 (soluble Vascuolar Cell Adhesion Molecule 1) ELISA Kit | MyBioSource MBS2515499 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine VCAM-1/CD106 (soluble Vascuolar Cell Adhesion Molecule 1) ELISA Kit

catalog :

MBS2515499

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2515499

products type :

ELISA Kit

products full name :

Porcine VCAM-1/CD106 (soluble Vascuolar Cell Adhesion Molecule 1) ELISA Kit

products short name :

[VCAM-1/CD106]

other names :

[vascular cell adhesion protein 1 isoform c; Vascular cell adhesion protein 1; vascular cell adhesion protein 1; CD106 antigen; vascular cell adhesion molecule 1; INCAM-100; CD_antigen: CD106]

products gene name :

[VCAM-1/CD106]

other gene names :

[VCAM1; VCAM1; CD106; INCAM-100; L1CAM; V-CAM 1; VCAM-1]

uniprot entry name :

VCAM1_HUMAN

reactivity :

Porcine

sequence length :

677

specificity :

This kit recognizes natural and recombinant Porcine VCAM-1/CD106. No significant cross-reactivity or interference between Porcine VCAM-1/CD106 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine VCAM-1/CD106 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VCAM-1/CD106. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VCAM-1/CD106. You can calculate the concentration of VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

315434271

ncbi acc num :

NP_001186763.1

ncbi gb acc num :

NM_001199834.1

uniprot acc num :

P19320

ncbi mol weight :

74,346 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Extracellular Matrix Organization Pathway (576262); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

uniprot summary :

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 1p32-p31. Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; apical part of cell; plasma membrane; podosome; sarcolemma; filopodium; external side of plasma membrane. Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding. Biological Process: response to nicotine; extracellular matrix organization and biogenesis; regulation of immune response; chronic inflammatory response; viral reproduction; heart development; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; response to nutrient; acute inflammatory response; aging

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975