catalog number :
MBS2515418
products type :
ELISA Kit
products full name :
Human GBP4 (Guanylate Binding Protein 4) ELISA Kit
products short name :
[GBP4]
other names :
[guanylate-binding protein 4; Guanylate-binding protein 4; guanylate-binding protein 4; GBP-4; GTP-binding protein 4; guanine nucleotide-binding protein 4; guanylate binding protein 4; GTP-binding protein 4; GBP-4; Guanine nucleotide-binding protein 4]
products gene name :
[GBP4]
other gene names :
[GBP4; GBP4; Mpa2; GBP-4]
uniprot entry name :
GBP4_HUMAN
specificity :
This kit recognizes Human GAD1 in samples. No Significant cross-reactivity or interference between Human GAD1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50 ng/mL. Sensitivity: 0.47 ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GAD1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GAD1 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GAD1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAD1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAD1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GAD1. You can calculate the concentration of Human GAD1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_443173.2
ncbi gb acc num :
NM_052941.4
ncbi mol weight :
73,165 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106)
ncbi summary :
Guanylate-binding proteins, such as GBP4, are induced by interferon and hydrolyze GTP to both GDP and GMP (Vestal, 2005 [PubMed 16108726]).[supplied by OMIM, Dec 2008]
uniprot summary :
GBP4: Binds GTP, GDP and GMP. Hydrolyzes GTP very efficiently; GDP rather than GMP is the major reaction product. Plays a role in erythroid differentiation. Belongs to the GBP family. Protein type: Cell adhesion; Vesicle; Hydrolase. Chromosomal Location of Human Ortholog: 1p22.2. Cellular Component: cytoplasm; nucleus. Molecular Function: GTPase activity; GTP binding. Biological Process: metabolic process
size5 :
10x96-Strip-Wells