catalog number :
MBS2515407
products type :
ELISA Kit
products full name :
Human sICAM-1/CD54 (Soluble Intercellular Adhesion Molecule 1) ELISA Kit
products short name :
[sICAM-1/CD54]
products gene name :
[sICAM-1/CD54]
specificity :
This kit recognizes natural and recombinant Human sICAM-1/CD54. No significant cross-reactivity or interference between Human sICAM-1/CD54 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human sICAM-1/CD54 concentrations in serum, plasma and other biological fluids.
products description :
. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sICAM-1/CD54. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of sICAM-1/CD54. You can calculate the concentration of sICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells