ELISA/assay

Human FAK (Focal Adhesion Kinase) ELISA Kit

MBS2515396

24-Strip-Wells

215 USD

ELISA Kit

Human FAK (Focal Adhesion Kinase) ELISA Kit

[FAK]

[focal adhesion kinase 1 isoform b; Focal adhesion kinase 1; focal adhesion kinase 1; FADK 1; FAK-related non-kinase polypeptide; PTK2 protein tyrosine kinase 2; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase-related nonkinase; protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK]

[FAK]

[PTK2; PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; FAK; FAK1; FADK 1; FRNK; PPP1R71]

FAK1_HUMAN

Human

1074

This kit recognizes Human FAAH in samples. No significant cross-reactivity or interference between HumanFAAH and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FAAH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FAAH. St andards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FAAH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FAAH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FAAH. You can calculate the concentration of Human FAAH in the samples by comparing the OD of the samples to the standard curve.

27886593

NP_005598.3

NM_005607.4

Q05397

114,253 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198806); Angiogenesis Pathway (198772); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed. Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Fak family. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; focal adhesion; lamellipodium; apical plasma membrane; cytoplasm; stress fiber; plasma membrane; microtubule organizing center; cell cortex; cytosol; nucleus. Molecular Function: JUN kinase binding; protein binding; signal transducer activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; SH2 domain binding; actin binding; protein kinase binding; ATP binding; receptor binding; protein kinase activity. Biological Process: heart morphogenesis; axon guidance; extracellular matrix organization and biogenesis; establishment of nucleus localization; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid autophosphorylation; neuron migration; cell motility involved in cell locomotion; negative regulation of synaptogenesis; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; vasculogenesis; cell structure disassembly during apoptosis; placenta development; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; platelet activation; central nervous system neuron axonogenesis; regulation of osteoblast differentiation; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; embryonic development; establishment of cell polarity; positive regulation of protein kinase activity; regulation of focal adhesion formation; endothelial cell migration; innate immune response; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; blood coagulation; vascular endothelial growth factor receptor signaling pathway; regulation of cytoskeleton organization and biogenesis; positive regulation of cell migration; negative regulation of apoptosis

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590