catalog number :
MBS2515385
products type :
ELISA Kit
products full name :
Human DNase-I (Deoxyribonuclease I) ELISA Kit
products short name :
[DNase-I]
other names :
[DNase I; Deoxyribonuclease-1; Deoxyribonuclease I; DNase I; INN: Dornase alfa]
products gene name :
[DNase-I]
other gene names :
[DNASE1; DNL1; DRNI; DNase I]
uniprot entry name :
DNAS1_HUMAN
specificity :
This kit recognizes HumanApoBin samples. No significantcross-reactivity or interference between HumanApoB and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000ng/mL. Sensitivity: 46.88ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level HumanApoB were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level HumanApoB were testedon 3 different plates, 20 replicates in eachplate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of HumanApoB concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HumanApoB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HumanApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HumanApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HumanApoB. You can calculate the concentration of HumanApoB in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAA63170.1
ncbi mol weight :
19,771 Da
uniprot summary :
DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family. Protein type: Motility/polarity/chemotaxis; EC 3.1.21.1; Secreted, signal peptide; Deoxyribonuclease; Secreted. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region; nuclear envelope; nucleus. Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity. Biological Process: apoptosis; DNA catabolic process, endonucleolytic. Disease: Systemic Lupus Erythematosus
size5 :
10x96-Strip-Wells