catalog number :
MBS2515369
products type :
ELISA Kit
products full name :
Human CA19-9 (Carbohydrate antigen19-9) ELISA Kit
products short name :
[CA19-9]
products gene name :
[CA19-9]
specificity :
This kit recognizes natural and recombinantHumanCA19-9. No significant cross-reactivity or interference between HumanCA19-9 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 6.25-400IU/mL. Sensitivity: Min: 3.75IU/mL; Max: 400IU/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanCA19-9concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CA19-9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CA19-9and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CA19-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CA19-9.You can calculate the concentration of CA19-9in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells