ELISA/assay

Human SEPP1(Selenoprotein P, Plasma)ELISA Kit

MBS2515309

48-Strip-Wells

410 USD

ELISA Kit

Human SEPP1(Selenoprotein P, Plasma)ELISA Kit

SEPP1

selenoprotein P isoform 2; Selenoprotein P; selenoprotein P; selenoprotein P, plasma, 1

SEPP1

SEPP1; SEPP1; SeP; SELP; SELP; SeP

SEPP1_HUMAN

Human

411

This kit recognizes natural and recombinant Human SEPP1. No significant cross-reactivity or interference between Human SEPP1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.094ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SEPP1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SEPP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SEPP1. You can calculate the concentration of SEPP1 in the samples by comparing the OD of the samples to the standard curve.

148277022

NP_001087195.1

NM_001093726.1

P49908

43,174 Da

Integrin-mediated Cell Adhesion Pathway (198816); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825)

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]

SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: extracellular space; extracellular region. Molecular Function: selenium binding. Biological Process: sexual reproduction; locomotory behavior; brain development; response to oxidative stress; selenium metabolic process; post-embryonic development; growth

48-Strip-Wells

410 USD

96-Strip-Wells

490