ELISA/assay

Human RNASET2 (Ribonuclease T2) ELISA Kit

MBS2515254

24-Strip-Wells

215 USD

ELISA Kit

Human RNASET2 (Ribonuclease T2) ELISA Kit

[RNASET2]

[ribonuclease T2; Ribonuclease T2; ribonuclease T2; ribonuclease 6; ribonuclease T2; Ribonuclease 6]

[RNASET2]

[RNASET2; RNASET2; RNASE6PL; bA514O12.3; RNASE6PL]

RNT2_HUMAN

Human

256

This kit recognizes Human RNASE3/ECP in samples. No significant cross-reactivity or interference between HumanRNASE3/ECPand analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 39.06-2500 pg/mL. Sensitivity: 23.44pg/mL.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human RNASE3/ECP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RNASE3/ECP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RNASE3/ECP and Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RNASE3/ECP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RNASE3/ECP. You can calculate the concentration of Human RNASE3/ECPin the samples by comparing the OD of the samples to the standard curve.

5231228

NP_003721.2

NM_003730.4

O00584

14,000 Da

This ribonuclease gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27, a region associated with human malignancies and chromosomal rearrangement. [provided by RefSeq, Jul 2008]

RNASET2: Has ribonuclease activity, with higher activity at acidic pH. May play a role in cellular RNA catabolism. Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM). An infantile- onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications. Belongs to the RNase T2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Ribonuclease; Secreted, signal peptide; RNA-binding; EC 3.1.27.-. Chromosomal Location of Human Ortholog: 6q27. Cellular Component: lysosomal lumen; extracellular space; endoplasmic reticulum lumen; lysosome; extracellular region. Molecular Function: ribonuclease T2 activity; RNA binding; ribonuclease activity. Biological Process: RNA catabolic process. Disease: Leukoencephalopathy, Cystic, Without Megalencephaly

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590