ELISA/assay

Monkey FGF21(Fibroblast Growth Factor 21) ELISA Kit

MBS2515207

24-Strip-Wells

235 USD

ELISA Kit

Monkey FGF21(Fibroblast Growth Factor 21) ELISA Kit

[FGF21]

[fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; fibroblast growth factor 21]

[FGF21]

[FGF21; FGF21; FGF-21]

FGF21_HUMAN

Monkey

209

This kit recognizes natural and recombinant Monkey FGF21. No significant cross-reactivity or interference between Monkey FGF21 and analogues was observed.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey FGF21 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF21. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FGF21. You can calculate the concentration of FGF21 in the samples by comparing the OD of the samples to the standard curve.

9506597

NP_061986.1

NM_019113.2

Q9NSA1

22,300 Da

MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction

24-Strip-Wells

235 USD

48-Strip-Wells

450

96-Strip-Wells

535

5x96-Strip-Wells

2255

10x96-Strip-Wells

3975