catalog number :
MBS2515085
products type :
ELISA Kit
products full name :
Porcine PAP (Plasmin-Antiplasmin Complex) ELISA Kit
products short name :
PAP
other names :
Pap; Pap; putative P1 acid phosphatase; Pap; Pap
other gene names :
pap; pap
uniprot entry name :
Q71T77_BPP1
specificity :
This kit recognizes natural and recombinant Porcine PAP. No significant cross-reactivity or interference between Porcine PAP and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Detection Range: 3.125-200ng/mL. Sensitivity: The minimum detectable dose of Porcine PAP is 1.875ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). Assay Type: Sandwich
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine PAP concentrations in serum, plasma and other biological fluids.
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PAP. You can calculate the concentration of PAP in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
YP_006560.1
ncbi gb acc num :
NC_005856.1
ncbi mol weight :
19,219 Da