catalog number :
MBS2515025
products type :
ELISA Kit
products full name :
Rat SDF-1 (Stromal Cell Derived Factor 1) ELISA Kit
products short name :
[SDF-1]
other names :
[stromal cell-derived factor 1 isoform alpha; Stromal cell-derived factor 1; stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); stromal-derived factor 1; xSDF-1; chemokine (C-X-C motif) ligand 12<; C-X-C motif chemokine 12]
products gene name :
[SDF-1]
other gene names :
[cxcl12; cxcl12; sdf1; sdf-1; xSDF-1; xSDF-1alpha; xSDF-1]
uniprot entry name :
SDF1_XENLA
specificity :
This kit recognizes Rat S100B in samples. No significant cross-reactivity or interference between Rat S100Band analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision withinan assay): 3 samples with low, midrange and high level Rat S100Bwere tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Rat S100Bwere testedon 3 different plates, 20 replicates in eachplate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat S100B concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat S100B. You can calculate the concentration of Rat S100B in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001165249.1
ncbi gb acc num :
NM_001171778.1
ncbi mol weight :
10,144 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (119282); Cytokine-cytokine Receptor Interaction Pathway (460); Intestinal Immune Network For IgA Production Pathway (128758); Intestinal Immune Network For IgA Production Pathway (128670)
uniprot summary :
Chemoattractant. Activates the C-X-C chemokine receptor cxcr4 to induce a rapid and transient rise in the level of intracellular calcium ions, and chemotaxis. Signaling with cxcr4 mediates the directional movement of mesodermal cells during gastrulation.
size5 :
10x96-Strip-Wells