catalog number :
MBS2515016
products type :
ELISA Kit
products full name :
Rat IL-16 (Interleukin 16) ELISA Kit
products short name :
[IL-16]
other names :
[IL16 protein; Pro-interleukin-16; pro-interleukin-16; lymphocyte chemoattractant factor; neuronal interleukin 16; prointerleukin 16; interleukin 16; Lymphocyte chemoattractant factor; LCF]
products gene name :
[IL-16]
other gene names :
[IL16; IL16; LCF; NIL16; PRIL16; prIL-16; IL-16; LCF]
uniprot entry name :
IL16_HUMAN
specificity :
This kit recognizes natural and recombinant Rat IL-16. No significant cross-reactivity or interference between Rat IL-16 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-16 concentrations in serum, plasma and other biological fluids.
products description :
. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-16. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-16. You can calculate the concentration of IL-16 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAI36661.1
ncbi mol weight :
59,345 Da
ncbi summary :
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
uniprot summary :
IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Motility/polarity/chemotaxis; DNA-binding; Cytokine. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus. Molecular Function: cytokine activity. Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; viral reproduction; leukocyte chemotaxis; immune response; induction of positive chemotaxis
size5 :
10x96-Strip-Wells