catalog number :
MBS2515011
products type :
ELISA Kit
products full name :
Sheep DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
products short name :
DHEA-S
products gene name :
DHEA-S
specificity :
This kit recognizes natural and recombinant Sheep DHEA-S. No significant cross-reactivity or interference between Sheep DHEA-S and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 7.813-500ng/mL. Sensitivity: The minimum detectable dose of Sheep DHEA-S is 4.688ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep DHEA-S concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DHEA-S. During the reaction, DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA-S. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.