ELISA/assay

Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

MBS2514900

96-Strip-Wells

445 USD

ELISA Kit

Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

SOD1

superoxide dismutase; Superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; Cu/Zn superoxide dismutase; SOD, soluble; epididymis secretory protein Li 44; indophenoloxidase A; superoxide dismutase, cystolic; superoxide dismutase 1, soluble; Superoxide dismutase 1; hSod1

SOD1

SOD1; SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer; hSod1

SODC_HUMAN

Rat

154

This kit recognizes natural and recombinant Rat SOD1. No significant cross-reactivity or interference between Rat SOD1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 0.156-10ng/mL. Sensitivity: 0.094ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat SOD1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

4507149

NP_000445.1

NM_000454.4

P00441

15,936 Da

AGE/RAGE Pathway (698754); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Dopamine Metabolism Pathway (920995); FOXA1 Transcription Factor Network Pathway (137979); Folate Metabolism Pathway (198833); Hemostasis Pathway (106028)

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family. Protein type: Nuclear receptor co-regulator; Mitochondrial; Apoptosis; EC 1.15.1.1; Oxidoreductase. Chromosomal Location of Human Ortholog: 21q22.11. Cellular Component: dendrite cytoplasm; extracellular space; protein complex; mitochondrion; extracellular region; mitochondrial intermembrane space; cytosol; nucleoplasm; extracellular matrix; cell soma; mitochondrial matrix; cytoplasm; plasma membrane; peroxisome; cytoplasmic vesicle; nucleus. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; chaperone binding; superoxide dismutase activity; Rac GTPase binding; protein phosphatase 2B binding. Biological Process: positive regulation of catalytic activity; activation of MAPK activity; cellular iron ion homeostasis; positive regulation of apoptosis; myeloid cell homeostasis; retrograde axon cargo transport; response to antibiotic; muscle maintenance; retinal homeostasis; glutathione metabolic process; neurofilament cytoskeleton organization and biogenesis; regulation of mitochondrial membrane potential; positive regulation of superoxide release; negative regulation of neuron apoptosis; placenta development; positive regulation of cytokine production; response to drug; platelet activation; cell aging; transmission of nerve impulse; regulation of organ growth; response to reactive oxygen species; response to ethanol; response to heat; heart contraction; superoxide release; relaxation of vascular smooth muscle; removal of superoxide radicals; locomotory behavior; response to organic substance; platelet degranulation; sensory perception of sound; ovarian follicle development; regulation of blood pressure; auditory receptor cell stereocilium organization and biogenesis; response to axon injury; anterograde axon cargo transport; negative regulation of cholesterol biosynthetic process; response to nutrient levels; response to superoxide; thymus development; regulation of T cell differentiation in the thymus; response to amphetamine; superoxide metabolic process; myelin maintenance in the peripheral nervous system; regulation of multicellular organism growth; response to hydrogen peroxide; response to copper ion; spermatogenesis; regulation of protein kinase activity; blood coagulation; embryo implantation; hydrogen peroxide biosynthetic process. Disease: Amyotrophic Lateral Sclerosis 1

96-Strip-Wells

445 USD